Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Jaundice

Jaundice

Jaundice, or icterus, is the yellow discoloration of the skin, sclerae, and mucous membranes. It happens when plasma bilirubin levels rise above 2.5-3 mg/dL, leading to bilirubin deposition in tissue.Bilirubin is a byproduct of hemoglobin degradation. In macrophages, hemoglobin breaks down into globin and heme. Globin is converted into amino acids, while heme is turned into biliverdin by heme oxygenase, which is then reduced to unconjugated bilirubin by biliverdin reductase.Unconjugated...

UV–Vis Spectroscopy of Conjugated Systems

UV–Vis Spectroscopy of Conjugated Systems

Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π&VeryThinSpace;→&VeryThinSpace;π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...

IR and UV–Vis Spectroscopy of Carboxylic Acids

IR and UV–Vis Spectroscopy of Carboxylic Acids

In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency, 1710 cm−1. The C=O bond of the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Developing new methods for the accurate evaluation of children's bone age based on X-ray images: a study on the accuracy and consistency of artificial intelligence and manual bone age evaluation.

Quantitative imaging in medicine and surgery·2026

Same author

Urban-rural difference and scale effect of ecosystem services of blue-green infrastructure.

Ying yong sheng tai xue bao = The journal of applied ecology·2025

Same author

Establishing prognostic models for intrahepatic cholangiocarcinoma based on immune cells.

World journal of gastrointestinal oncology·2024

Same author

The tumor immune microenvironment of nasopharyngeal carcinoma after gemcitabine plus cisplatin treatment.

Nature medicine·2023

Same author

Symbiotic Fungi Alter the Acquisition of Phosphorus in <i>Camellia oleifera</i> through Regulating Root Architecture, Plant Phosphate Transporter Gene Expressions and Soil Phosphatase Activities.

Journal of fungi (Basel, Switzerland)·2022

Same author

A Novel Sensor for Undrained Shear Strength Measurement in Very Soft to Soft Marine Sediments Based on Optical Frequency Domain Reflectometry Technology.

Sensors (Basel, Switzerland)·2022

Search research articles

Related Experiment Video

Updated: May 15, 2026

Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation

Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation

Published on: June 4, 2015

Spectroscopic studies on bilirubin aggregate at liquid/liquid interface.

Zhi-Yan Xiao¹, Jian-Hua Yin

¹Department of Radiation Oncology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Analytical and Bioanalytical Chemistry

|January 16, 2013

Summary

Researchers detected bilirubin (BR) aggregation at liquid interfaces using FTIR and UV-Vis spectroscopy. This aggregation alters BR molecular structure and hydrogen bonding, impacting its biological interactions.

More Related Videos

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Published on: September 12, 2019

Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses

Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses

Published on: September 11, 2023

Related Experiment Videos

Last Updated: May 15, 2026

Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation

Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation

Published on: June 4, 2015

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Published on: September 12, 2019

Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses

Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses

Published on: September 11, 2023

Area of Science:

Biochemistry
Spectroscopy
Materials Science

Background:

Bilirubin (BR) is a crucial biological molecule with implications in various physiological and pathological processes.
Understanding the aggregation behavior of BR is essential for elucidating its role in diseases like jaundice and its interactions within biological systems.

Purpose of the Study:

To detect and characterize bilirubin aggregation at liquid/liquid interfaces.
To investigate the structural and conformational changes of bilirubin upon aggregation using advanced spectroscopic techniques.

Main Methods:

Utilized Fourier Transform Infrared (FTIR) imaging/spectroscopy to analyze molecular structure.
Employed Ultraviolet-Visible (UV/Vis) absorption spectroscopy to monitor spectral changes indicative of aggregation.
Investigated bilirubin aggregation at liquid/liquid interfaces.

Main Results:

Successfully detected bilirubin aggregation at liquid interfaces for the first time.
Observed a new shoulder at 474 nm and a red shift to 497 nm in UV-Vis spectra, confirming interface-induced aggregation.
FTIR analysis revealed significant changes in hydrogen-bond-sensitive bands, indicating broken intramolecular hydrogen bonds and altered molecular structure.

Conclusions:

The study provides novel insights into bilirubin aggregation mechanisms at interfaces.
The observed structural changes, including altered hydrogen bonding and conformation, are critical for understanding BR's biological functions.
These findings have implications for studying BR interactions with biomolecules, its transport, and its pathological effects on tissues.