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Updated: May 15, 2026

Postoperative Ileus Murine Model
04:26

Postoperative Ileus Murine Model

Published on: July 12, 2024

Novel method for studying postoperative ileus in mice.

Sjoerd Hw van Bree1, Andrea Nemethova, Fleur S van Bovenkamp

  • 1Tytgat institute of Liver and Intestinal Research, Department of Gastroenterology & Hepatology, Academic Medical Center Amsterdam, the Netherlands.

International Journal of Physiology, Pathophysiology and Pharmacology
|January 16, 2013
PubMed
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Researchers developed a standardized device to apply fixed pressure during intestinal manipulation, creating a reproducible preclinical model for postoperative ileus (POI). This improved model reduces variability in studying gastrointestinal dysfunction after surgery.

Area of Science:

  • Gastroenterology
  • Surgical Research
  • Preclinical Modeling

Background:

  • Postoperative ileus (POI) is a common complication after abdominal surgery, characterized by transient gastrointestinal motility inhibition.
  • Current preclinical models using intestinal manipulation lack standardization, leading to inconsistent research findings.
  • A novel device was developed to apply standardized, adjustable pressure during intestinal manipulation for POI modeling.

Purpose of the Study:

  • To develop and validate a standardized device for applying controlled pressure during intestinal manipulation.
  • To establish a reproducible preclinical model for studying postoperative ileus (POI).
  • To compare the new standardized method with conventional manipulation techniques.

Main Methods:

Keywords:
Gastrointestinal motility and physiologyinflammationintestinal transitmicepostoperative ileus

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Published on: September 11, 2012

A Mouse Model of Intestinal Partial Obstruction
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Functional Assessment of Intestinal Motility and Gut Wall Inflammation in Rodents: Analyses in a Standardized Model of Intestinal Manipulation
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Published on: September 11, 2012

A Mouse Model of Intestinal Partial Obstruction
07:33

A Mouse Model of Intestinal Partial Obstruction

Published on: March 5, 2018

  • A purpose-designed device was used to apply graded, standardized pressure during intestinal manipulation in a mouse model.
  • Gastrointestinal (GI) transit was measured using fluorescein isothiocyanate (FITC)-labeled dextran.
  • Intestinal inflammation was assessed by quantifying myeloperoxidase positive cells and cytokine levels (IL-6, IL-1β, MCP-1).

    • Main Results:

    • Increasing applied pressure led to a dose-dependent reduction in GI transit.
    • Intestinal inflammation, characterized by leukocyte infiltration and elevated pro-inflammatory cytokines, was observed with increasing pressure.
    • Applying 9 grams of pressure yielded consistent delays in intestinal transit with reduced intra-individual variation compared to conventional methods.

    Conclusions:

    • The standardized pressure manipulation method provides a reproducible and low-variation model for POI research.
    • This validated model can be used to investigate the pathophysiology of POI.
    • The model is suitable for evaluating the efficacy of novel anti-inflammatory strategies for POI.