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[Analyze on the expression differences of key genes in saponins biosynthesize in Eleutherococcus senticosus and its
Zhao-Bin Xing1, Peng Wu, Yue-Hong Long
1College of Life Science, Hebei United University, Tangshan 063000, China. xingzhaobin@yahoo.com.cn
Objective:
To analyze the expression differences of SS, SE and bAS in leaf, petiole and callus from leaf and petiole of Eleutherococcus senticosus.
Methods:
Calluses were induced from explants of E. senticosus leaf and petiole. The expression amount of SS, SE and bAS gene was detected by real time PCR.
Results:
The expression amount of SS, SE and bAS genes in callus from leaf were 89.3%, 73.8% and 83.4% of that in leaf, respectively. The expression amount of SS, SE and bAS genes in callus from petiole were 80.2%, 87.7% and 70.9% of that in petiole, respectively.
Conclusion:
The expression amount of SS, SE and bAS gene in callus from E. senticosus are significantly lower than that in plant.
