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Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

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Related Experiment Video

Updated: May 15, 2026

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Visualizing movement in the tumor microenvironment landscape.

Veronica Calvo, Paraic A Kenny

    Breast Cancer Research : BCR
    |January 17, 2013
    PubMed
    Summary

    The extracellular matrix impacts tumor growth. A laminin-rich basement membrane limited invasion, while a collagen I matrix promoted tumor cell migration and spread, highlighting the matrix's role in controlling cancer dissemination.

    Area of Science:

    • Oncology
    • Cell Biology
    • Biomaterials Science

    Background:

    • The tumor microenvironment, particularly the extracellular matrix (ECM), significantly influences cancer progression and metastasis.
    • Understanding how ECM composition affects tumor cell behavior is crucial for developing effective cancer therapies.

    Purpose of the Study:

    • To investigate the impact of different extracellular matrix compositions on tumor cell invasion and dissemination.
    • To elucidate the role of the basement membrane versus interstitial stroma in modulating mammary tumor cell behavior.

    Main Methods:

    • Primary human and mouse mammary tumor explants were cultured in vitro.
    • Tumor fragments were cultured within a three-dimensional laminin-rich basement membrane matrix.
    • Identical tumor fragments were cultured within a collagen I matrix, mimicking the interstitial breast stroma.

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    A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment
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    Main Results:

    • Culture within the laminin-rich basement membrane matrix resulted in minimal tumor cell invasion.
    • Tumor fragments cultured in the collagen I matrix showed pronounced protrusive migration and local dissemination.
    • These findings indicate that ECM composition dictates the mode and extent of tumor cell invasion.

    Conclusions:

    • The laminin-rich basement membrane acts as a barrier, constraining tumor cell invasion.
    • The collagen I-rich interstitial stroma facilitates tumor cell migration and local spread.
    • ECM composition is a critical determinant of tumor progression and metastasis.