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Related Experiment Video

Updated: May 15, 2026

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

Trisomy 8: a common finding in mouse embryonic stem (ES) cell lines.

Young Mi Kim1, Ji-Yun Lee, Lijun Xia

  • 1Department of Pediatrics, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. shibo-li@ouhsc.edu.

Molecular Cytogenetics
|January 17, 2013
PubMed
Summary

Chromosomal abnormalities in embryonic stem (ES) cells correlate with poor germ line transmission. Analyzing chromosomes improves gene targeting experiments in mice.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • Generating transgenic and knockout mice is crucial for studying gene function.
  • Embryonic stem (ES) cells are key for creating genetically modified mice but often show inefficient germ line transmission.

Purpose of the Study:

  • To investigate the correlation between chromosomal anomalies and the efficiency of germ line transmission in ES cells.
  • To identify potential causes for the inefficiency in gene targeting experiments.

Main Methods:

  • Conventional cytogenetic analysis was performed on 97 constructed ES cell lines.
  • Fluorescence in situ hybridization (FISH) was utilized to detect chromosomal abnormalities.

Main Results:

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Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation
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Related Experiment Videos

Last Updated: May 15, 2026

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation
09:14

Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation

Published on: June 14, 2024

  • Chromosomal abnormalities were detected in 44% of the analyzed ES cell lines.
  • Common anomalies included trisomy 8, mosaic trisomy 8, and partial trisomy 8 due to unbalanced translocations.

    • Conclusions:

    • Chromosomal analysis is essential for enhancing the success rate and quality of gene targeting experiments.
    • Identifying and addressing chromosomal abnormalities in ES cells can improve germ line transmission efficiency.