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Rapid technique for cryopreservation of Echinococcus multilocularis metacestodes
S Bretagne1, F Beaujean, A Uner
1Laboratoire de Parasitologie, Faculté de Médecine, Creteil, France.
International Journal for Parasitology
|April 1, 1990
Summary
Successful cryopreservation of Echinococcus multilocularis isolates was achieved using a controlled-rate freezer and liquid nitrogen storage. This method maintained the viability of all tested parasite samples, ensuring their availability for future research.
Area of Science:
- Parasitology
- Cryobiology
- Molecular Biology
Background:
- Echinococcus multilocularis is a significant human pathogen.
- Maintaining parasite viability is crucial for research and diagnostics.
- Cryopreservation offers a method for long-term storage of biological samples.
Purpose of the Study:
- To establish a reliable cryopreservation protocol for Echinococcus multilocularis cysts.
- To assess the viability of cryopreserved E. multilocularis isolates.
Main Methods:
- E. multilocularis cysts were homogenized.
- Glycerol was added to a final concentration of 10% as a cryoprotectant.
- Samples were cryopreserved using a controlled-rate freezer at 1°C min-1 and stored in liquid nitrogen.
Main Results:
- All 22 tested isolates of E. multilocularis were successfully cryopreserved.
- Viability of the parasite was maintained after cryopreservation and storage.
Conclusions:
- The developed method is effective for cryopreserving E. multilocularis.
- This technique ensures the long-term availability of viable parasite isolates for scientific study.