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Compartmentalization of lipid peroxidation in sepsis by multidrug-resistant gram-negative bacteria: experimental and

Chryssoula Toufekoula, Vassileios Papadakis, Thomas Tsaganos

    Critical Care (London, England)
    |January 18, 2013
    PubMed
    Abstract

    Insights

    Lipid peroxidation, measured by malondialdehyde (MDA), is compartmentalized in sepsis caused by multidrug-resistant Gram-negative bacteria. MDA levels varied across organs in rats and correlated with specific organ failures in human sepsis patients.

    Area of Science:

    • Biochemistry
    • Pathophysiology
    • Microbiology

    Background:

    • Lipid peroxidation is increasingly linked to organ failure in sepsis.
    • No prior studies have investigated this in sepsis caused by multidrug-resistant (MDR) Gram-negative bacteria.

    Purpose of the Study:

    • To investigate the role and localization of lipid peroxidation in sepsis induced by MDR Gram-negative bacteria.
    • To compare findings in an animal model with human sepsis patients.

    Main Methods:

    • Sepsis was induced in rats using Pseudomonas aeruginosa (MDR isolate).
    • Malondialdehyde (MDA) levels were measured in rat tissues and human patient serum using thiobarbiturate assay and HPLC.
    • Human data included patients with ventilator-associated pneumonia (VAP) and sepsis; serum MDA was tracked over 7 days.

    Main Results:

    • Septic rats showed increased MDA in the liver, spleen, and aorta, but decreased MDA in the kidney.
    • Human patients with hepatic dysfunction and ARDS had higher serum MDA; those with renal dysfunction had lower MDA.
    • MDA levels in survivors indicated different patterns for hepatic/ARDS versus renal dysfunction.

    Conclusions:

    • Animal and human results suggest compartmentalization of lipid peroxidation during systemic infections by MDR Gram-negative bacteria.
    • MDA serves as a potential biomarker for specific organ dysfunction in this context.

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