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Related Experiment Video

Updated: May 15, 2026

Microfluidic Model of Necrotizing Enterocolitis Incorporating Human Neonatal Intestinal Enteroids and a Dysbiotic Microbiome
06:51

Microfluidic Model of Necrotizing Enterocolitis Incorporating Human Neonatal Intestinal Enteroids and a Dysbiotic Microbiome

Published on: July 28, 2023

Intestinal proteome changes during infant necrotizing enterocolitis.

Pingping Jiang1, Birgitte Smith, Niels Qvist

  • 1School of Biological Sciences, University of Hong Kong, Hong Kong, China.

Pediatric Research
|January 18, 2013
PubMed
Summary

Necrotizing enterocolitis (NEC) alters protein expression in the gut, with heat shock proteins (HSPs) significant in the small intestine. Secretory proteins show promise as biomarkers for NEC progression.

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Last Updated: May 15, 2026

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Area of Science:

  • Gastroenterology
  • Proteomics
  • Biomarker Discovery

Background:

  • Necrotizing enterocolitis (NEC) is a severe gastrointestinal disease in infants.
  • Understanding proteomic changes in NEC is crucial for identifying biomarkers and therapeutic targets.

Purpose of the Study:

  • To compare the proteome of NEC-affected intestinal and colonic tissues with near-normal tissues.
  • To identify differentially expressed proteins and pathways involved in NEC.
  • To explore potential biomarkers for NEC progression.

Main Methods:

  • Gel-based proteomics was used to analyze protein expression in human intestinal and colonic samples.
  • Western blot and immunohistochemistry validated proteomic findings.
  • Comparison was made between NEC-affected and adjacent healthy tissues from the same patients.

Main Results:

  • Differential expression of 30 proteins in the small intestine and 23 in the colon was observed.
  • Histamine receptors, actins, globins, immunoglobulin, and antitrypsin were commonly affected.
  • Heat shock proteins (HSPs) were notably altered in the small intestine.
  • Proteins involved in antioxidation, angiogenesis, cytoskeleton, and metabolism were affected.
  • Secretory proteins like antitrypsin and haptoglobin differed significantly in NEC tissues.

Conclusions:

  • NEC impacts distinct molecular pathways in the small intestine and colon.
  • Heat shock proteins (HSPs) may play a critical role in small intestine NEC.
  • Investigating identified secretory proteins as circulating NEC biomarkers is recommended.