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Updated: May 15, 2026

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method
Published on: May 8, 2014
Lipidome and proteome map of myelin membranes
Gopakumar Gopalakrishnan1, Anshul Awasthi, Wiam Belkaid
1Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada. gopakumar.gopalakrishnan@mcgill.ca
Abstract:
To understand the molecular anatomy of myelin membranes, we performed a large-scale, liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS)-based lipidome and proteome screen on freshly purified human and murine myelin fractions. We identified more than 700 lipid moieties and above 1,000 proteins in the two species, including 284 common lipids and 257 common proteins. This study establishes the first comprehensive map of myelin membrane components in human and mice. Although this study demonstrates many similarities between human and murine myelin, several components have been identified exclusively in each species. Future quantitative validation studies focused on interspecies differences will authenticate the myelin membrane anatomy. The combined lipidome and proteome map presented here can nevertheless be used as a reference library for myelin health and disease.
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