Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 15, 2026

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Bioengineered skin humanized model of psoriasis.

Marta Carretero¹, Sara Guerrero-Aspizua, Marcela Del Río

¹Epithelial Biomedicine Division, Basic Research Department, Centro de Investigaciones Energéticas, Medioambientales, y Tecnológicas, Centre for Biomedical Research on Rare Diseases U714, Madrid, Spain.

Methods in Molecular Biology (Clifton, N.J.)

|January 18, 2013

Summary

Researchers developed a reproducible bioengineered human skin mouse model for psoriasis. This model allows for studying disease mechanisms and evaluating potential psoriasis therapies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

MesenSistem-EB: systemic haploidentical mesenchymal stem cell therapy in recessive dystrophic epidermolysis bullosa associated with clinical benefits and correlated with MCP1 and sCD40L dynamics.

Frontiers in immunology·2026

Same author

Zinc as a Biomarker of Nutritional Status and Clinical Burden in Recessive Dystrophic Epidermolysis Bullosa: Implications for Preventive Monitoring.

Nutrients·2026

Same author

Base edited skin equivalents with clinical potential for ex vivo correction of recessive dystrophic epidermolysis bullosa.

Molecular therapy : the journal of the American Society of Gene Therapy·2025

Same author

Correction: Mathematical models and computational approaches in CAR-T therapeutics.

Frontiers in immunology·2025

Same author

Sephardic origins revealed for rare skin disorder, recessive dystrophic epidermolysis bullosa, in individuals carrying the unique c.6527insC mutation.

Journal of medical genetics·2025

Same author

Mathematical models and computational approaches in CAR-T therapeutics.

Frontiers in immunology·2025

Area of Science:

Biomedical Engineering
Immunology
Dermatology

Background:

Psoriasis is a chronic inflammatory skin disease with complex pathogenesis.
Existing animal models often fail to fully recapitulate human psoriasis.
There is a need for robust in vivo models to study disease mechanisms and test therapeutics.

Purpose of the Study:

To describe a novel bioengineered human skin mouse model for psoriasis.
To establish a reproducible and scalable platform for psoriasis research.
To facilitate the evaluation of therapeutic compounds for psoriasis.

Main Methods:

Generation of a humanized mouse model by engrafting human skin onto immunodeficient mice.
Induction of psoriatic phenotype through intradermal administration of T helper 1 (Th1) lymphocytes and Th17 recombinant cytokines.

More Related Videos

Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System

Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System

Published on: October 17, 2025

Related Experiment Videos

Last Updated: May 15, 2026

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System

Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System

Published on: October 17, 2025

Mild skin barrier disruption using tape-stripping to enhance engraftment and phenotype development.

Utilized both allogeneic and autologous cell sources from healthy donors and psoriatic patients.

Main Results:

Achieved a large and homogenous number of engrafted animals with human skin.
Successfully induced a psoriatic phenotype in the human skin grafts.
Demonstrated the model's reproducibility and scalability.
Confirmed the model's utility in both allogeneic and autologous settings.

Conclusions:

The developed skin-humanized mouse model is a powerful tool for investigating psoriasis pathogenesis.
This model offers a feasible platform for preclinical evaluation of psoriasis therapeutics.
The bioengineering approach provides a reproducible method for generating disease-specific models.