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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: May 15, 2026

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
07:29

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Published on: May 16, 2020

RNAi-mediated gene function analysis in skin.

Slobodan Beronja1, Elaine Fuchs

  • 1Laboratory of Mammalian Cell Biology & Development, Howard Hughes Medical Institute, The Rockefeller University, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 18, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a new RNA interference (RNAi) method for analyzing gene function in mouse skin. This technique enables rapid, efficient, and stable gene knockdown for studying epidermal biology.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Analyzing gene function in skin is crucial for understanding epidermal biology.
  • Existing methods may be time-consuming or inefficient for rapid analysis.

Purpose of the Study:

  • To develop a novel RNAi-mediated method for efficient gene function analysis in mouse skin.
  • To provide a faster and more accessible tool for studying epidermal development and disease.

Main Methods:

  • Ultrasound-guided in utero microinjection of lentivirus into the amniotic cavity of embryonic day 9 mice.
  • Utilizing short hairpin RNA (shRNA) for single-gene function analysis via knockdown.
  • Rapid transduction into mouse skin for subsequent phenotypic analysis.

Main Results:

  • Achieved rapid, efficient, and stable gene transduction into mouse skin.
  • Demonstrated the utility of the technique for single-gene function analysis through shRNA-mediated knockdown.
  • The method simplifies experimental setup, requiring no animal mating and allowing analysis within days.

Conclusions:

  • The developed RNAi method offers a powerful and versatile tool for skin biology research.
  • This technique significantly expands the genetic and molecular toolbox for investigating epidermal functions.
  • It enables accelerated functional studies, facilitating quicker insights into skin development and disease mechanisms.