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Electron microscope detection of an endogenous infection of retrovirus-like particles in L20B cells
Jason A Roberts1, Bruce R Thorley, Leesa D Bruggink
1Victorian Infectious Diseases Reference Laboratory, 10 Wreckyn Street, North Melbourne, Victoria 3051, Australia.
Abstract:
L20B cells are a cell line commonly used for the isolation of poliovirus. The current study indicates that L20B cells are chronically infected with a retrovirus-like particle that replicates in the cytoplasm and buds through the plasma membrane. The findings indicate that care is needed in the use of L20B cells for certain virus isolation studies and emphasize the importance of electron microscope studies as an adjunct to the development of diagnostic virology protocols.
Insights
L20B cells, used for poliovirus isolation, are chronically infected with a retrovirus-like particle. This discovery necessitates caution in their use for virus isolation and highlights the value of electron microscopy in virology.
Area of Science:
- Virology
- Cell Biology
- Microbiology
Background:
- L20B cells are a standard cell line for poliovirus isolation.
- Retrovirus-like particles have been identified within L20B cells.
Discussion:
- The identified retrovirus-like particles replicate in the cytoplasm.
- These particles bud through the plasma membrane of L20B cells.
- The presence of these particles may impact poliovirus isolation studies.
Key Insights:
- L20B cells harbor a chronic retrovirus-like particle infection.
- Replication occurs in the cytoplasm and egress via plasma membrane budding.
- This finding has implications for poliovirus research.
Outlook:
- Careful consideration is required when using L20B cells for specific virus isolation.
- Electron microscopy is crucial for developing accurate diagnostic virology protocols.
- Further research may elucidate the nature and impact of this particle.
