Identification of Bioaerosols Released from an Egg Production Facility in the Southeast United States

This field study investigated biological characteristics of aerosols emitted from a commercial egg production farm (layer operation). Bioaerosol samples were taken on this farm at five sampling locations covering emission source (inside a layer barn) and four ambient surrounding stations at four wind directions. All-glass impingers (AGI) were used for the field sampling. AGI fluid samples were plated in duplicate on Trypticase Soy Agar for growth of bacteria and Sabouraud Dextrose Agar for growth of fungi. The most prominent bacterial colony types were identified using a combination of methods that include recording characteristics of colony morphology; performing a Gram staining method and metabolic analyses using the Biolog system. Results from thirty-five AGI samples taken at the five stations through seven sampling events over four seasons indicate that there were significantly lower total bacterial concentrations in the samples collected from ambient stations as compared with the samples collected in the layer house; the mean bacterial concentration at the in-house sampling station was 3.86×10(5)±1.74×10(5) cfu/m(3), whereas the mean bacterial concentrations at four ambient stations in the vicinity of the farm ranged from 1.3×10(3) to 6.2×10(3) cfu/m(3) with no significant differences in mean among ambient stations. There were also no significant differences in fungi concentrations among all sampling stations. Mean fungi concentrations at the in-house station was 3.0×10(3)±4.45×10(3) cfu/m(3), whereas the mean concentrations at the ambient stations ranged from 7.4×10(3) to 1.7×10(4) cfu/m(3). The most prominent bacterial species differed among all five stations. Three of the most prominent bacteria from samples taken at all five stations were gram positive. Fungal type also differed from station to station.