A novel approach to enhancing ganoderic acid production by Ganoderma lucidum using apoptosis induction
Bang-Jau You1, Miin-Huey Lee, Ni Tien
1Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan. bangjau@mail.cmu.edu.tw
Plos One
|January 18, 2013
Summary
Inducing apoptosis in Ganoderma lucidum with aspirin significantly increases ganoderic acid (GA) production. This novel approach boosts active compound yields, offering a new method for fungal secondary metabolite enhancement.
Area of Science:
- Mycology
- Biochemistry
- Pharmacology
Background:
- Ganoderma lucidum is a widely used medicinal mushroom in Asia.
- Ganoderic acids (GAs) are key bioactive compounds in G. lucidum.
- Understanding GA biosynthesis regulation is crucial for optimizing its medicinal use.
Purpose of the Study:
- To investigate the role of apoptosis signaling in GA biosynthesis.
- To explore apoptosis induction as a novel strategy for enhancing GA production.
Main Methods:
- Aspirin was used to induce apoptosis in G. lucidum.
- Apoptosis was confirmed via TUNEL assay and nuclear morphology observation.
- Ganoderic acid production was quantified using HPLC.
Main Results:
- Aspirin treatment induced G. lucidum cell apoptosis.
- Maximum induction of ganoderic acid 24 (GA24) and total GAs reached 2.7-fold and 2.8-fold, respectively, within 1 day.
- Apoptosis induction led to higher GA yields compared to standard 1.5-month culture.
Conclusions:
- Fungal apoptosis is linked to GA biosynthesis.
- Apoptosis induction is a viable method to enhance secondary metabolite production in fungi.
- ROS accumulation and Hog-1 phosphorylation may be involved in GA regulation.
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