Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 15, 2026

Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification
09:17

Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification

Published on: July 12, 2019

Modulating the physical microenvironment to study regenerative processes in vitro using cells from mouse phalangeal

Kristen M Lynch1, Tabassum Ahsan

  • 1Department of Biomedical Engineering, Tulane University, New Orleans, Louisiana 70118, USA.

Tissue Engineering. Part A
|January 19, 2013
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vertical growth pattern as a determinant of mandibular asymmetry.

Pakistan journal of medical sciences·2022
Same author

Strategies for scalable manufacturing and translation of MSC-derived extracellular vesicles.

Stem cell research·2020
Same author

Bioreactor Parameters for Microcarrier-Based Human MSC Expansion under Xeno-Free Conditions in a Vertical-Wheel System.

Bioengineering (Basel, Switzerland)·2020
Same author

Modulation of the in vitro angiogenic potential of human mesenchymal stromal cells from different tissue sources.

Journal of cellular physiology·2020
Same author

Peak MSC-Are We There Yet?

Frontiers in medicine·2018
Same author

Actin and myosin II modulate differentiation of pluripotent stem cells.

PloS one·2018
Same journal

A Novel 3D Bioprinting Strategy for Bioengineering of Urethra with Clinical Relevance.

Tissue engineering. Part A·2026
Same journal

Hydrogel-Encapsulated Primed MSCs Enhance Regeneration in Full-Thickness Porcine Burn Wounds.

Tissue engineering. Part A·2026
Same journal

Unidirectional Porous Carbonate Apatite Fabricated by Gelatin-Based Freeze Casting for Bone Regeneration.

Tissue engineering. Part A·2026
Same journal

Regenerative Nanoscaffolds for Chronic Tympanic Membrane Perforation: From Bench to Clinical Translation.

Tissue engineering. Part A·2026
Same journal

Impact of IFN-γ-Pretreated Umbilical Cord Mesenchymal Stem Cells Implanted in Mesh on Pelvic Organ Prolapse.

Tissue engineering. Part A·2026
Same journal

The Driving Force of Hierarchical Collagen Fiber Formation: A Review of Tendon, Ligament, and Meniscus Mechanobiology.

Tissue engineering. Part A·2026
See all related articles

Murine phalangeal (P2) cells are more migratory and generate greater traction forces than distal (P3) cells, which are more proliferative, offering insights into digit regeneration and in vitro models.

Area of Science:

  • Regenerative Medicine
  • Cell Biology
  • Tissue Engineering

Background:

  • Human epimorphic regeneration of complex tissues is limited, unlike in some animal models.
  • Mouse models show level-specific regeneration, with distal phalangeal elements regenerating but more proximal ones not.
  • Stromal cells from proximal (P2) and distal (P3) murine phalangeal elements offer a model to study differences in regenerative potential.

Purpose of the Study:

  • To compare the cellular responses of proximal (P2) and distal (P3) murine phalangeal stromal cells to injury.
  • To investigate how different in vitro culture conditions (adherent, suspension, collagen gel) influence cell behavior.
  • To characterize inherent differences in P2 and P3 cells relevant to wound healing and regeneration.

Main Methods:

More Related Videos

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

Related Experiment Videos

Last Updated: May 15, 2026

Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification
09:17

Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification

Published on: July 12, 2019

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

  • Adherent, suspension, and collagen gel cultures were used to assess P2 and P3 stromal cell behavior.
  • Cell migration, collagen gel compaction, and proliferation rates were measured.
  • Expression of key cytoskeletal and keratin proteins was analyzed.

    • Main Results:

    • P2 cells exhibited higher migration and collagen gel compaction than P3 cells, linked to greater cytoskeletal protein expression.
    • P3 cells demonstrated increased proliferation compared to P2 cells under all culture conditions.
    • P3 cells showed enhanced proliferation and matrix gene expression in suspension culture, suggesting its relevance to blastema-like interactions.

    Conclusions:

    • Stromal cells from different levels of the murine digit possess distinct inherent properties influencing their response to the physical microenvironment.
    • P2 cells' migratory and force-generating capacities are relevant to wound healing models like collagen gel compaction.
    • P3 cells' proliferative response in suspension culture suggests its utility for studying blastema formation and promoting regeneration.