Related Experiment Video
Updated: May 15, 2026
Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
Published on: October 21, 2022
Metabolic profile of keratoconic cornea
Tomasz Kryczka1, Niels Ehlers, Kim Nielsen
1Department of Neuroscience, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway. kryczka.tomasz@gmail.com
Purpose:
To investigate the difference in metabolic profile of keratoconic and normal corneas using two different analysis methods.
Methods:
Keratoconic corneas were obtained from patients (aged 19-27) during transplantation surgery. Control samples were obtained from human donors (aged 61-75) 1-8 h post-mortem. The metabolic profile of tissues was investigated with high-resolution magic angle spinning (1)H nuclear magnetic resonance (NMR) spectroscopy and high performance liquid chromatography (HPLC).
Results:
Nine amino acids and 20 metabolites were assigned with HPLC and NMR spectroscopy, respectively. No significant biochemical difference was revealed between keratoconic and control samples, which represent distant age groups.
Conclusions:
It suggests that development of keratoconus might be related to the accelerated ageing of the cornea. This issue warrants further studies.
More Related Videos
07:29Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis
Published on: November 12, 2015
11:29Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies
Published on: January 3, 2011