Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scent and adhesion drive insect dispersal of environmental yeast.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Antibody fine specificity correlates with protection from malaria for the RTS,S vaccine in young African children: A post hoc analysis of a phase IIb randomised controlled trial.

PLoS medicine·2026
Same author

Timing of antiretroviral treatment initiation and seropositivity to measles virus among children living with HIV in rural Zambia.

medRxiv : the preprint server for health sciences·2026
Same author

Evidence of a putative new species Haemagogus 'Trinidad sp. A' from the Caribbean sharing mitogenome lineages with species endemic to the Amazon.

Scientific reports·2026
Same author

Species composition and blood feeding patterns of understudied Anopheles mosquitoes in southern Zambia, an area of low malaria transmission.

Malaria journal·2026
Same author

Low-density asymptomatic parasitemia in southern Zambia does not lead to clinical malaria and is not associated with household transmission: results from a two-year cohort study.

Research square·2026

Related Experiment Video

Updated: May 15, 2026

A Simple Chelex Protocol for DNA Extraction from Anopheles spp.
07:16

A Simple Chelex Protocol for DNA Extraction from Anopheles spp.

Published on: January 9, 2013

A simple Chelex protocol for DNA extraction from Anopheles spp.

Mulenga Musapa1, Taida Kumwenda, Mtawa Mkulama

  • 1Malaria Institute at Macha.

Journal of Visualized Experiments : Jove
|January 19, 2013
PubMed
Summary

A new Chelex-based DNA extraction method offers a simple, affordable alternative for malaria surveillance. This technique is comparable to standard methods, requiring fewer reagents and less time, making it ideal for resource-limited settings.

More Related Videos

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing
05:34

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

Published on: April 15, 2021

Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations
05:51

Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations

Published on: June 28, 2021

Related Experiment Videos

Last Updated: May 15, 2026

A Simple Chelex Protocol for DNA Extraction from Anopheles spp.
07:16

A Simple Chelex Protocol for DNA Extraction from Anopheles spp.

Published on: January 9, 2013

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing
05:34

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

Published on: April 15, 2021

Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations
05:51

Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations

Published on: June 28, 2021

Area of Science:

  • Molecular biology
  • Entomology
  • Parasitology

Background:

  • Molecular tools are crucial for malaria surveillance and control programs in endemic countries.
  • Sustainable malaria control requires simple, affordable methods with minimal reagent and equipment needs.
  • Existing DNA extraction methods can be time-consuming and require numerous reagents.

Purpose of the Study:

  • To present a simplified Chelex-based DNA extraction technique for malaria parasite and vector DNA.
  • To evaluate the efficacy of the Chelex method compared to a standard salting out protocol.
  • To determine the suitability of the Chelex method for resource-limited settings.

Main Methods:

  • Morphological identification of 72 Anopheles gambiae sl. mosquitoes.
  • Dissection of mosquito head/thorax and abdomen.
  • DNA extraction using simplified Chelex or standard salting out protocols.
  • PCR amplification for DNA quality (ND4 gene), sibling species identification, and Plasmodium falciparum typing.

Main Results:

  • The Chelex method demonstrated high sensitivity and specificity across all PCR applications compared to the salting out protocol.
  • Chelex method required only three reagents and 37 minutes, versus 10 reagents and 2 hours 47 minutes for salting out.
  • No significant differences were observed in amplicon signal proportions between the two methods.
  • The Chelex approach showed 93% sensitivity and 82% specificity for DNA quality PCR.

Conclusions:

  • The Chelex-based DNA extraction method is a viable, efficient alternative to the standard salting out protocol.
  • This simplified method is comparable in performance and significantly reduces time and reagent requirements.
  • The Chelex method is a sustainable and cost-effective solution for malaria surveillance in resource-limited settings.