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Assessment of vascular permeability increase in the mouse by dye leakage during paw edema
1Department of Pharmacology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Abstract:
Vascular permeability increase induced by histamine, bradykinin, platelet-activating factor (PAF), or phorbol myristate acetate (PMA) in the mouse paw was assessed by the dye leakage method. The amount of dye extracted from the paw showed a clear dose-response relationship to the dose of each agonist injected into the paw. Among the autacoids used, PAF showed the most potent activity in the mouse paw. The results are consistent with those seen in the rat dorsal skin as previously reported. Involvement of histamine, 5-hydroxytryptamine and PAF is suggested in the vascular permeability increase induced by PMA in the mouse paw.
Insights
Platelet-activating factor (PAF) most potently increased vascular permeability in mouse paws, as measured by dye leakage. Histamine, bradykinin, and PMA also induced permeability increases, with PMA involving histamine, serotonin, and PAF.
Area of Science:
- Pharmacology
- Immunology
- Inflammation research
Background:
- Vascular permeability is a key indicator of inflammation.
- Histamine, bradykinin, platelet-activating factor (PAF), and phorbol myristate acetate (PMA) are known mediators of vascular permeability.
- Understanding the specific roles of these mediators in different models is crucial for developing targeted anti-inflammatory therapies.
Purpose of the Study:
- To quantify and compare the vascular permeability-inducing effects of histamine, bradykinin, PAF, and PMA in the mouse paw.
- To investigate the potential involvement of histamine, serotonin, and PAF in PMA-induced vascular permeability in the mouse paw.
- To compare findings in the mouse paw model with previously reported data in the rat dorsal skin model.
Main Methods:
- Vascular permeability was assessed in mouse paws using a dye leakage method.
- Different doses of histamine, bradykinin, PAF, and PMA were injected into the mouse paw.
- Dye extravasation was quantified by extracting and measuring the dye from paw tissue.
Main Results:
- A clear dose-response relationship was observed for all tested agonists (histamine, bradykinin, PAF, PMA).
- Platelet-activating factor (PAF) demonstrated the most potent vascular permeability-increasing activity in the mouse paw among the tested autacoids.
- The results in the mouse paw model were consistent with previous findings in the rat dorsal skin.
- PMA-induced vascular permeability in the mouse paw suggested the involvement of histamine, 5-hydroxytryptamine (serotonin), and PAF.
Conclusions:
- PAF is a potent inducer of vascular permeability in the mouse paw.
- The mouse paw model effectively reflects inflammatory responses observed in other species, like rats.
- PMA-induced inflammation involves multiple mediators, including histamine, serotonin, and PAF, highlighting complex inflammatory pathways.