Multiplexed surface plasmon resonance imaging for protein biomarker analysis

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Proteomics

Background:

• Reliable detection of ligand and analyte binding is crucial for medical diagnostics.
• Advances in proteomics necessitate advanced techniques for identifying protein biomarkers in complex samples.
• Surface Plasmon Resonance Imaging (SPRi) offers label-free, real-time detection of molecular interactions.

Purpose of the Study:

• To detail the fabrication of a novel SPR imaging instrument.
• To demonstrate the instrument's capability for analyzing molecular binding interactions.
• To enable multiplexed analysis and biomarker identification from complex biological samples.

Main Methods:

• Fabrication of a high-density microfluidic SPRi chip.
• Utilizing various immobilization chemistries for targeted analyte capture.
• Implementing controlled recovery of bound biomarkers for subsequent mass spectrometry analysis.

Main Results:

• Demonstrated successful fabrication and operation of the microfluidic SPRi instrument.
• Showcased controlled recovery of bound biomarkers for identification.
• Successfully identified activated leukocyte cell adhesion molecule (ALCAM) from human crude cell lysates.

Conclusions:

• The developed microfluidic SPRi instrument provides a reliable, label-free method for detecting molecular binding.
• The system enables multiplexed analysis and biomarker identification from complex biological samples.
• This technology holds significant potential for advancing medical diagnostics and cancer biomarker research.