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Updated: May 15, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Multiplexed surface plasmon resonance imaging for protein biomarker analysis.

Eric Ouellet¹, Louise Lund, Eric T Lagally

¹Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|January 19, 2013

Summary

This study presents a novel microfluidic Surface Plasmon Resonance Imaging (SPRi) instrument for sensitive, label-free detection of molecular binding. The system successfully identified the cancer biomarker activated leukocyte cell adhesion molecule (ALCAM) in human cell lysates.

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Area of Science:

Biomedical Engineering
Analytical Chemistry
Proteomics

Background:

Reliable detection of ligand and analyte binding is crucial for medical diagnostics.
Advances in proteomics necessitate advanced techniques for identifying protein biomarkers in complex samples.
Surface Plasmon Resonance Imaging (SPRi) offers label-free, real-time detection of molecular interactions.

Purpose of the Study:

To detail the fabrication of a novel SPR imaging instrument.
To demonstrate the instrument's capability for analyzing molecular binding interactions.
To enable multiplexed analysis and biomarker identification from complex biological samples.

Main Methods:

Fabrication of a high-density microfluidic SPRi chip.
Utilizing various immobilization chemistries for targeted analyte capture.

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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

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Implementing controlled recovery of bound biomarkers for subsequent mass spectrometry analysis.

Main Results:

Demonstrated successful fabrication and operation of the microfluidic SPRi instrument.
Showcased controlled recovery of bound biomarkers for identification.
Successfully identified activated leukocyte cell adhesion molecule (ALCAM) from human crude cell lysates.

Conclusions:

The developed microfluidic SPRi instrument provides a reliable, label-free method for detecting molecular binding.
The system enables multiplexed analysis and biomarker identification from complex biological samples.
This technology holds significant potential for advancing medical diagnostics and cancer biomarker research.