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Related Concept Videos

Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

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Related Experiment Video

Updated: May 15, 2026

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
12:59

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

In vitro-derived gametes from stem cells.

Franklin D West1, Reza Shirazi, Pezhman Mardanpour

  • 1Regenerative Bioscience Center, University of Georgia, Athens, GA, USA.

Seminars in Reproductive Medicine
|January 19, 2013
PubMed
Summary

Generating functional sperm cells from stem cells offers new hope for treating male infertility. These in vitro-derived (IVD) sperm can fertilize eggs, leading to live offspring and advancing reproductive medicine.

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Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

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Related Experiment Videos

Last Updated: May 15, 2026

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
12:59

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm
08:08

Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm

Published on: November 19, 2020

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro
09:47

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

Published on: July 16, 2013

Area of Science:

  • Reproductive Biology
  • Stem Cell Research
  • Infertility Therapeutics

Background:

  • Male infertility, often due to lack of sperm production, affects a significant portion of reproductive-age couples.
  • Assisted reproductive technologies (ART) have advanced infertility treatment, but rely on available gametes.
  • Azoospermia, the absence of sperm, presents a major challenge in male infertility.

Purpose of the Study:

  • To explore the potential of generating functional gametes in vitro for treating infertility.
  • To investigate the feasibility of producing sperm cells from stem cells for reproductive purposes.
  • To establish a novel therapeutic approach for azoospermic men.

Main Methods:

  • Generation of germ cells from mouse embryonic stem cells.
  • Development and characterization of in vitro-derived (IVD) germ cells.
  • Assessment of IVD sperm fertilization capacity and subsequent offspring viability.

Main Results:

  • Successful generation of mature haploid sperm cells from embryonic stem cells.
  • Demonstration that IVD sperm are capable of fertilizing eggs.
  • Production of live offspring resulting from fertilization with IVD sperm.

Conclusions:

  • In vitro-derived gametes represent a significant breakthrough in overcoming infertility, particularly for azoospermic individuals.
  • Advancements in IVD gametogenesis will reshape the future of infertility treatment and open new therapeutic avenues.
  • IVD gametogenesis provides a valuable model system for studying sperm cell development and related biological processes.