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Related Experiment Video

Updated: May 15, 2026

In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time
09:19

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Published on: May 24, 2020

Hemogenic endothelium in a dish.

Michael Kyba1

  • 1Lillehei Heart Institute and Department of Pediatrics, University of Minnesota.

Blood
|January 19, 2013
PubMed
Summary

Researchers identified Sox17 as a key regulator of hemogenic endothelium development using human stem cells. Conditional Sox17 expression helps capture endothelial cells during the critical hemogenic transition phase.

Area of Science:

  • Hematopoiesis and stem cell biology
  • Endothelial cell differentiation
  • Developmental biology

Background:

  • Hemogenic endothelium is a transient vascular structure that gives rise to hematopoietic stem cells.
  • Understanding the molecular mechanisms regulating hemogenic endothelium development is crucial for regenerative medicine and understanding blood disorders.

Purpose of the Study:

  • To identify novel regulators of hemogenic endothelium development.
  • To investigate the role of Sox17 in endothelial cell fate determination during hematopoiesis.
  • To develop a method for capturing endothelial cells at the hemogenic transition stage.

Main Methods:

  • Utilized human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells.
  • Employed conditional gene expression systems to manipulate Sox17 levels.

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Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Published on: June 16, 2019

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Last Updated: May 15, 2026

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Published on: May 24, 2020

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Isolation of Murine Embryonic Hemogenic Endothelial Cells

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  • Analyzed endothelial cell populations and their hematopoietic potential through cell sorting and functional assays.

    • Main Results:

    • Sox17 was identified as a critical regulator of hemogenic endothelium specification.
    • Conditional expression of Sox17 enabled the capture of endothelial cells undergoing hemogenic transition.
    • This approach provides insights into the dynamic process of blood cell formation from endothelial precursors.

    Conclusions:

    • Sox17 plays a pivotal role in the transition of endothelial cells to a hemogenic state.
    • The findings offer a new tool for studying early hematopoiesis and identifying therapeutic targets for blood-related diseases.