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Updated: May 15, 2026

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
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Glucose signalling positively regulates aliphatic glucosinolate biosynthesis.

Huiying Miao1, Jia Wei, Yanting Zhao

  • 1Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou, China.

Journal of Experimental Botany
|January 19, 2013
PubMed
Summary

Glucose significantly boosts aliphatic glucosinolate production in Arabidopsis thaliana by activating key genes like MYB28 and MYB29. This process is modulated by glucose signaling pathways involving HXK1 and RGS1.

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Area of Science:

  • Plant molecular biology
  • Plant biochemistry

Background:

  • Aliphatic glucosinolates are crucial plant defense compounds.
  • Glucose signaling pathways regulate various plant metabolic processes.

Purpose of the Study:

  • To investigate the role of glucose in aliphatic glucosinolate biosynthesis in Arabidopsis thaliana.
  • To identify the key regulatory factors involved in glucose-mediated glucosinolate production.

Main Methods:

  • Utilized Arabidopsis thaliana mutants in glucosinolate biosynthesis, regulation, and glucose signaling pathways.
  • Analyzed aliphatic glucosinolate content and gene expression levels (MYB28, MYB29, CYP79F1, CYP79F2).

Main Results:

  • Glucose treatment significantly increased aliphatic glucosinolate levels and the expression of MYB28 and MYB29.
  • The glucose-induced increase in glucosinolates and gene expression was abolished in the myb28myb29 double mutant.
  • Glucose-insensitive mutants (gin2-1, abi5-7, rgs1-2) showed reduced glucosinolate content and MYB gene expression.

Conclusions:

  • Glucose promotes aliphatic glucosinolate biosynthesis in Arabidopsis thaliana.
  • MYB28 and MYB29 act synergistically, with MYB28 being dominant, in glucose-induced glucosinolate production.
  • Glucose signaling pathways mediated by HXK1 and RGS1, involving transcription factors MYB28, MYB29, and ABI5, regulate this process.