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Updated: May 15, 2026
A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells
Published on: August 7, 2017
Methods of detection of mesenchymal stem cells in the kidneys during therapy of experimental renal pathologies
E Yu Plotnikov1, N V Pul'kova, D N Silachev
1A. N. Belozerskii Institute of Physicochemical Biology, M. V. Lomonosov Moscow Medical University, Moscow, Russia.
Abstract:
We studied the possibility of using different methods for identification of mesenchymal multipotent stromal cells in the renal parenchyma under conditions of experimental thermal ischemia of the kidneys and acute pyelonephritis. In vivo and in vitro methods of identification of mesenchymal multipotent stromal cells by magnetic resonance imaging and immunological and immunohistochemical methods were compared. Labeling of stem cells with iron-containing particles followed by their histological identification and immunohistochemical staining with species-specific antibodies were the most informative methods. Active migration of the cells to the renal tissue was detected by these methods in experimental acute pyelonephritis with inflammation foci.
Insights
Identifying mesenchymal multipotent stromal cells in kidney tissue was studied. Iron-labeled cell tracking via histology and immunohistochemistry proved most effective for observing cell migration in experimental kidney conditions.
Area of Science:
- Regenerative Medicine
- Cell Biology
- Nephrology
Background:
- Mesenchymal multipotent stromal cells (MSCs) hold therapeutic potential for kidney diseases.
- Accurate identification and tracking of MSCs in renal tissue are crucial for evaluating treatment efficacy.
- Experimental models of kidney injury, such as thermal ischemia and pyelonephritis, are essential for studying MSC behavior.
Purpose of the Study:
- To compare different methods for identifying mesenchymal multipotent stromal cells (MSCs) within the renal parenchyma.
- To evaluate the efficacy of in vivo and in vitro techniques, including magnetic resonance imaging (MRI) and immunological/immunohistochemical methods.
- To determine the most informative approach for tracking MSCs in experimental kidney conditions.
Main Methods:
- Comparison of in vivo and in vitro identification techniques for MSCs.
- Utilized magnetic resonance imaging (MRI) for cell visualization.
- Employed immunological and immunohistochemical methods for cell characterization.
- Investigated labeling of stem cells with iron-containing particles for enhanced detection.
- Performed histological identification and species-specific immunohistochemical staining.
Main Results:
- Labeling MSCs with iron-containing particles followed by histological and immunohistochemical identification was the most informative method.
- Demonstrated active migration of labeled MSCs to renal tissue.
- Observed cell migration specifically in experimental acute pyelonephritis, correlating with inflammation foci.
- Magnetic resonance imaging (MRI) and other immunological methods showed limitations in precise identification compared to the chosen approach.
Conclusions:
- Iron-labeling combined with histological and immunohistochemical analysis provides a highly informative method for tracking mesenchymal multipotent stromal cells (MSCs) in renal parenchyma.
- This approach successfully detected active MSC migration to sites of inflammation in experimental acute pyelonephritis.
- The findings support the utility of advanced cell tracking techniques for understanding MSC behavior in kidney injury models.
