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Protein-protein Interfaces

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...

Protein-Protein Interfaces

Protein-Protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...

Protein Networks

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

Protein Networks

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

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Related Experiment Video

Updated: May 15, 2026

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

Plant protein interactomes.

Pascal Braun¹, Sébastien Aubourg, Jelle Van Leene

¹Department of Plant Systems Biology, Center for Life and Food Sciences Weihenstephan, Technische Universität München (TUM), 85354 Freising-Weihenstephan, Germany. pbraun@wzw.tum.de

Annual Review of Plant Biology

|January 22, 2013

Summary

This summary is machine-generated.

Understanding plant protein interactions is key to discovering protein functions. This review covers experimental and computational methods for mapping plant protein interactome networks, aiding biological discovery.

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Identification of Post-translational Modifications of Plant Protein Complexes

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Last Updated: May 15, 2026

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

Area of Science:

Plant Biology
Molecular Biology
Bioinformatics

Background:

Protein-protein interactions (PPIs) are fundamental to biological processes.
Analyzing PPIs reveals protein functions and complex biological networks.
Large-scale studies are crucial for understanding plant protein interactions.

Purpose of the Study:

To review major approaches for constructing, analyzing, and validating plant protein interactome networks.
To highlight experimental and computational methods for large-scale PPI mapping.
To discuss bioinformatics resources and findings from recent plant interactome studies.

Main Methods:

Experimental approaches: Binary methods (e.g., yeast two-hybrid) for direct interactions.
Experimental approaches: Affinity purification coupled with mass spectrometry for protein complexes.
Computational approaches: Bioinformatics predictions for identifying potential interactions.

Main Results:

Overview of established and emerging techniques for plant PPI mapping.
Discussion of data quality control measures for interactome networks.
Integration of experimental and computational data for comprehensive network analysis.

Conclusions:

Effective construction and analysis of plant protein interactome networks are essential for functional genomics.
A combination of experimental and computational methods provides a robust approach to PPI studies.
Recent large-scale interactome maps offer valuable insights into plant molecular mechanisms.