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Related Experiment Video

Updated: May 15, 2026

Tissue Engineering of the Intestine in a Murine Model

Tissue Engineering of the Intestine in a Murine Model

Published on: December 1, 2012

Human tissue-engineered small intestine forms from postnatal progenitor cells.

Daniel E Levin¹, Erik R Barthel, Allison L Speer

¹Division of Pediatric Surgery, Children's Hospital Los Angeles, Keck School of Medicine of the University of Southern California, Los Angeles, CA 90027, USA.

Journal of Pediatric Surgery

|January 22, 2013

Summary

This summary is machine-generated.

This study demonstrates the successful creation of human tissue-engineered small intestine (TESI) from postnatal tissue. This breakthrough in regenerative medicine offers new hope for treating short bowel syndrome (SBS).

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Last Updated: May 15, 2026

Tissue Engineering of the Intestine in a Murine Model

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Published on: December 1, 2012

Innervation of Human Intestinal Organoids

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Published on: January 17, 2025

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Published on: July 9, 2021

Area of Science:

Regenerative Medicine
Tissue Engineering
Gastroenterology

Background:

Short bowel syndrome (SBS) is a debilitating condition requiring novel treatments.
Previous attempts at tissue-engineered small intestine (TESI) primarily used fetal tissues.
Human postnatal tissue has not been successfully used for TESI xenografts previously.

Purpose of the Study:

To report the first successful creation of human TESI from postnatal tissue.
To evaluate the survival, growth, and differentiation of human organoid units (OU) on scaffolds in a murine model.

Main Methods:

Human small bowel resection specimens were used to create organoid units (OU).
OU were loaded onto biodegradable scaffolds and implanted into immunodeficient mice.
TESI was harvested after 4 weeks and analyzed via immunostaining for specific cell types and tissue structures.

Main Results:

All generated TESI was confirmed to be of human origin.
Immunofluorescence revealed the presence of all four differentiated small intestinal cell types (enterocytes, Paneth cells, enteroendocrine cells, goblet cells).
Muscularis, subepithelial myofibroblasts, and nerve tissue were also identified in the engineered construct.

Conclusions:

The developed technique enables the survival, growth, and differentiation of human postnatal small intestinal OU into full-thickness TESI.
This regenerative medicine approach shows promise for future therapeutic strategies for SBS.