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Increased expression of dectin-1 in nasal polyps
Jian Gong1, Pei Wang, Ze-heng Qiu
1Department of Otorhinolaryngology-Head and Neck Surgery, Sun Yat-sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, P.R. China.
Purpose:
A fungal etiology has been proposed to underlie severe nasal polyps (NP). Dectin-1 is an innate immune pattern recognition receptor which is involved in the recognition of some pathogenic fungi. We investigated the Dectin-1 levels in NP in order to evaluate the implication of such expression with respect to the development of NP.
Materials And Methods:
Normal inferior turbinate tissues were obtained from forty patients undergoing surgery for augmentation rhinoplasty. Nasal polyp tissues were obtained from 53 patients who underwent endoscopic sinus surgery for chronic polypoid rhinosinusitis. Real-time polymerase chain reaction and Western blot analysis were performed to evaluate the mRNA and protein level of Dectin-1, respectively. ELISA was carried out to evaluate the cytokine production (IL-4, IL-5, IL-10, and TNF-α) in NP.
Results:
Real-time polymerase chain reaction and Western-blot analysis showed that Dectin-1 expression in NP was increased compared with that in normal nasal inferior turbinate tissues. ELISA results suggest that the local expression of type-1 and type-2 inflammatory cytokine is skewed toward type-2 inflammatory cytokine in NP.
Conclusions:
These results suggest that Dectin-1 may play a role in the development of NP, and the production of Dectin-1, IL-4 and IL-5 (type-2 cytokines), may mainly participate in the inflammatory reaction in NP.
Insights
Increased Dectin-1 expression in nasal polyps (NP) suggests a role in fungal-related inflammation. This finding highlights Dectin-1 and type-2 cytokines in the inflammatory response within NP.
Area of Science:
- Immunology
- Otolaryngology
- Mycology
Background:
- Nasal polyps (NP) may have a fungal cause.
- Dectin-1 is an innate immune receptor recognizing fungi.
- Investigating Dectin-1 in NP is crucial for understanding its development.
Purpose of the Study:
- To assess Dectin-1 levels in nasal polyps.
- To correlate Dectin-1 expression with NP development.
- To explore the role of Dectin-1 in NP pathogenesis.
Main Methods:
- Compared Dectin-1 mRNA and protein in NP tissues versus normal turbinates.
- Utilized Real-time PCR and Western blot for Dectin-1 analysis.
- Measured IL-4, IL-5, IL-10, and TNF-α cytokine levels via ELISA.
Main Results:
- Dectin-1 expression was significantly higher in NP tissues.
- Inflammatory cytokine profiles in NP were skewed towards type-2.
- Elevated Dectin-1 correlated with type-2 inflammatory markers.
Conclusions:
- Dectin-1 may contribute to the pathogenesis of nasal polyps.
- Dectin-1, IL-4, and IL-5 likely play key roles in NP inflammation.
- Findings support a fungal-immune interaction in NP development.
