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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 15, 2026

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

Comprehending dynamic protein methylation with mass spectrometry.

Leila Afjehi-Sadat1, Benjamin A Garcia

  • 1Epigenetics Program, Perelman School of Medicine, University of Pennsylvania, 1009C Stellar-Chance Laboratories, 422 Curie Boulevard, Philadelphia, PA 19104, USA.

Current Opinion in Chemical Biology
|January 22, 2013
PubMed
Summary

Protein methylation, a key post-translational modification, is studied using mass spectrometry. This review covers mass spectrometry methods for identifying, quantifying, and analyzing protein methylation dynamics.

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Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
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Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

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Last Updated: May 15, 2026

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
08:48

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Protein methylation is a crucial post-translational modification (PTM) impacting numerous cellular processes.
  • Methylation occurs on various amino acids, including arginine and lysine, catalyzed by methyltransferases.
  • Understanding protein methylation is vital for deciphering cellular functions and disease mechanisms.

Purpose of the Study:

  • To review the applications of mass spectrometry (MS) technologies for studying protein methylation.
  • To highlight methods for identifying, quantifying, and characterizing protein methylation.
  • To discuss strategies for measuring dynamic and differential in vivo protein methylation rates.

Main Methods:

  • Mass spectrometry (MS)-based techniques are the primary methods discussed.
  • The review covers different MS approaches for PTM analysis.
  • Metabolic labeling strategies coupled with MS are presented for quantitative analysis.

Main Results:

  • Mass spectrometry is a powerful and sensitive tool for protein methylation studies.
  • Various MS techniques enable comprehensive characterization of protein methylation.
  • In vivo methylation rates can be dynamically measured using MS and metabolic labeling.

Conclusions:

  • Mass spectrometry is indispensable for advancing the field of protein methylation research.
  • The discussed MS applications provide insights into the functional roles of protein methylation.
  • Future research can leverage these techniques to explore methylation in various biological contexts.