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Published on: November 22, 2024
Sequence polymorphism in the Trypanosoma rangeli HSP70 coding genes allows typing of the parasite KP1(+) and KP1(-)
Claudia Cuervo1, M Carmen Thomas, Manuel C López
1Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad Ciencias, Pontificia Universidad Javeriana, Carrera 7 No. 43-82, Edificio 50, Laboratorio 113, Bogotá, Colombia. claudia.cuervo@javeriana.edu.co
Abstract:
The genes encoding the Trypanosoma rangeli heat shock protein 70kDa were sequenced and their genomic organization determined. This human parasite has medical relevance as it shares antigens, hosts and geographical regions with the etiological agent of Chagas' disease, Trypanosoma cruzi. The T. rangeli HSP70 genes are highly conserved regarding their tandem organization, and deduced amino acid sequences among T. rangeli KP1(+) and KP1(-) groups and other trypanosomatids. Nevertheless, a variable number of the immunogenic GMPG motif was observed among HSP70 copies within the same T. rangeli isolate and among different isolates. Interestingly, a polymorphism at nucleotide level affecting the SphI restriction site allowed the differentiation of KP1(-) and KP1(+) groups.

