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Updated: May 15, 2026

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
Published on: July 6, 2022
Mitochondrial DNA variations in ova and blastocyst: implications in assisted reproduction
Monis Bilal Shamsi1, Periyasamy Govindaraj, Latika Chawla
1Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science, New Delhi, India.
Abstract:
Mitochondrial DNA (mtDNA) of oocyte is critical for its function, embryo quality and development. Analysis of complete mtDNA of 49 oocytes and 18 blastocysts from 67 females opting for IVF revealed 437 nucleotide variations. 40.29% samples had either disease associated or non-synonymous novel or pathogenic mutation in evolutionarily conserved regions. Samples with disease associated mtDNA mutations had low fertilization rate and poor embryo quality, however no difference in implantation or clinical pregnancy rate was observed. Screening mtDNA from oocyte/blastocyst is a simple, clinically reliable method for diagnostic evaluation of female infertility and may reduce risk of mtDNA disease transmission.
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