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Modified-Release Drug Delivery Systems: Stimuli-Activated01:30

Modified-Release Drug Delivery Systems: Stimuli-Activated

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Related Experiment Video

Updated: May 15, 2026

Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers
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Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers

Published on: August 12, 2014

Bioactive coronary stent coating based on layer-by-layer technology for siRNA release.

S Hossfeld1, A Nolte, H Hartmann

  • 1NMI-Natural and Medical Sciences Institute at the University of Tuebingen, Reutlingen, Germany.

Acta Biomaterialia
|January 22, 2013
PubMed
Summary
This summary is machine-generated.

This study developed drug-eluting stents with small interfering RNA (siRNA) nanoplexes using layer-by-layer technology for improved vascular regeneration. The innovative stent coatings demonstrated effective siRNA delivery to cells and artery walls with good hemocompatibility.

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Last Updated: May 15, 2026

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Published on: September 18, 2015

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Cardiovascular Research

Background:

  • Percutaneous transluminal coronary angioplasty (PTCA) is used to treat stenotic coronary arteries.
  • Drug-eluting stents (DESs) improve outcomes but can be enhanced for better vascular regeneration.
  • Small interfering RNA (siRNA) delivery via stents is a promising approach.

Purpose of the Study:

  • To develop and evaluate layer-by-layer (LbL) coated coronary stents incorporating siRNA nanoplexes (NPs).
  • To assess the stability, cellular uptake, and ex vivo delivery of siRNA NPs from the stent coatings.
  • To evaluate the hemocompatibility of the developed bioactive stent coatings.

Main Methods:

  • Fabrication of hyaluronic acid/chitosan (HA/Chi) multilayer films with incorporated chitosan-siRNA NPs using LbL technology.
  • Characterization of coating thickness and stability using quartz crystal microbalance, fluorescence microscopy, and scanning electron microscopy.
  • In vitro assessment of NP uptake by endothelial cells and ex vivo NP transfer into porcine artery walls; hemocompatibility testing.

Main Results:

  • Homogeneous HA/Chi and Chi-siRNA NP coatings were successfully fabricated on stents.
  • Coatings remained stable after sterilization and stent expansion.
  • In vitro studies showed endothelial cell uptake of NPs, and ex vivo studies demonstrated NP transfer into porcine artery walls.
  • The developed coatings exhibited good hemocompatibility.

Conclusions:

  • Chitosan-siRNA NPs can be effectively incorporated into HA/Chi multilayer films using LbL technology.
  • The developed coatings facilitate siRNA NP delivery to endothelial cells in vitro and artery walls ex vivo.
  • These bioactive coatings show potential as an innovative tool for coronary stent applications.