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Updated: May 15, 2026

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Published on: November 1, 2018
[Epidemiological situation and the E gene evolution of dengue virus in Guangzhou, 2011]
Li-yun Jiang1, Yi-min Cao, Yang Xu
1Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
Objective:
To investigate the epidemiological characteristics of Dengue and the E gene of the new isolated strains.
Methods:
Epidemiological data and serum samples were collected. Serotypes were detected by real-time PCR and virus was isolated in C6/36. E gene of the new isolated strains were sequenced and analyzed by Mega 4.0.
Results:
The cases of Dengue reached at the peak during September and November, with Serotype 1, 2 and 4 were involved. Five strains of serotype l were isolated, with 4 of them fell into the clad of Asia genotype, and 1 belonged to America/Africa genotype.
Conclusion:
The strains isolated in Guangzhou showed a high identity to the Southeast Asian strains. There seemed high risk of outbreak of Dengue in this area, However, the Dengue virus might have already been localized.
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