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Sorafenib induces autophagy and suppresses activation of human macrophage

Jiunn-Chang Lin1, Chien-Liang Liu, Jie-Jen Lee

  • 1Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.

International Immunopharmacology
|January 23, 2013
PubMed
Abstract

Insights

Sorafenib, a cancer drug, induces autophagy and alters human macrophage function. This study reveals its impact on innate immunity cells, affecting their viability and antigen expression.

Area of Science:

  • Immunology
  • Cell Biology
  • Pharmacology

Background:

  • Sorafenib is a multi-kinase inhibitor used for advanced cancers.
  • It is known to modulate dendritic cells.
  • This study investigates sorafenib's effects on macrophages, key innate immunity cells.

Purpose of the Study:

  • To examine the effects of sorafenib on human macrophages.
  • To understand sorafenib's impact on macrophage autophagy and function.

Main Methods:

  • Macrophages were derived from human peripheral blood monocytes.
  • Cell viability, surface antigens, and autophagy markers (LC-3B lipidation, autophagosome accumulation) were assessed.
  • Macrophage function (co-stimulatory molecule expression, phagocytosis, ROS production, cytokine secretion) was evaluated.

Main Results:

  • Sorafenib inhibited macrophage viability and induced autophagy.
  • Autophagy was confirmed by morphological changes and molecular markers.
  • Sorafenib reduced co-stimulatory molecule CD80 expression, phagocytosis, ROS production, and IL-10 secretion.

Conclusions:

  • Sorafenib induces autophagy in human macrophages.
  • Sorafenib modulates human macrophage function, impacting their immune response.
  • These findings highlight sorafenib's dual role as a therapeutic agent and immune modulator.

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