Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of the ZEB1-BCL2 axis in cancer-associated fibroblasts as a strategy to improve chemotherapy response in intrahepatic cholangiocarcinoma.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Environmental risk ranking of 20 metal(loid)s in urban runoff: A Swedish nationwide meta-analysis.

Journal of hazardous materials·2026
Same author

Veterinary Medicine Students' Perceptions of Hunting and Game Meat: A Cross-Sectional Survey at a Portuguese University.

Animals : an open access journal from MDPI·2026
Same author

Digitalisation and automation in smart biomanufacturing: uncertainties, challenges and early pathways towards safe and sustainable design.

New biotechnology·2026
Same author

Hematology in times of crisis: Lessons learned and strategic priorities from the EHA-ASH Joint Workshop on Ukraine.

HemaSphere·2026
Same author

Semi-automated isolation of parenchymal and non-parenchymal liver cells from mice and humans with enhanced stellate cell fraction.

Cell & bioscience·2026

Related Experiment Video

Updated: May 14, 2026

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology
12:36

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

Published on: March 14, 2020

Distinct dedifferentiation processes affect caveolin-1 expression in hepatocytes.

Christoph Meyer1, Johanna Dzieran, Yan Liu

  • 1Department of Medicine II, Section Molecular Hepatology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany. steven.dooley@medma.uni-heidelberg.de.

Cell Communication and Signaling : CCS
|January 24, 2013
PubMed
Summary
This summary is machine-generated.

Hepatocyte dedifferentiation in culture differs from TGF-β driven dedifferentiation. Caveolin-1 (Cav1) is not a reliable epithelial mesenchymal transition (EMT) marker, especially in TGF-β mediated processes.

More Related Videos

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro
10:12

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Published on: December 20, 2019

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells
09:15

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells

Published on: July 18, 2019

Related Experiment Videos

Last Updated: May 14, 2026

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology
12:36

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

Published on: March 14, 2020

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro
10:12

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Published on: December 20, 2019

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells
09:15

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells

Published on: July 18, 2019

Area of Science:

  • Cell Biology
  • Hepatology
  • Molecular Biology

Background:

  • Hepatocyte dedifferentiation and loss of polarity in primary culture impede metabolic analyses.
  • Transforming growth factor-beta (TGF-β) is a profibrotic cytokine that induces epithelial mesenchymal transition (EMT) in liver cells.
  • Distinguishing culture-induced dedifferentiation from TGF-β-driven dedifferentiation is crucial.

Purpose of the Study:

  • To differentiate between culture-dependent and TGF-β-induced hepatocyte dedifferentiation.
  • To elucidate the molecular pathways involved in these processes.
  • To evaluate caveolin-1 (Cav1) as a potential marker for EMT in hepatocytes.

Main Methods:

  • Comparative analysis of hepatocyte dedifferentiation under culture conditions versus TGF-β stimulation.
  • Investigation of signaling pathways including FAK/Src/ERK/AKT.
  • Assessment of mesenchymal markers, E-Cadherin, Snai1, and Cav1 expression.
  • Analysis of Cav1 expression in well-differentiated and poorly differentiated hepatocellular carcinoma (HCC) cell lines.

Main Results:

  • Both culture and TGF-β induce mesenchymal markers, but Snai1 upregulation and E-Cadherin downregulation are specific to TGF-β.
  • Culture-dependent dedifferentiation involves FAK/Src/ERK/AKT pathways and induces caveolin-1 (Cav1).
  • Cav1 is not upregulated in TGF-β-mediated hepatocyte EMT, questioning its validity as an EMT marker. HCC cell lines show differential Cav1 expression patterns.

Conclusions:

  • A clear distinction is established between intrinsic hepatocyte dedifferentiation and TGF-β-mediated dedifferentiation.
  • The study elucidates the specific cellular pathways driving these distinct dedifferentiation processes.
  • Caveolin-1 (Cav1) is demonstrated to be an unreliable marker for epithelial mesenchymal transition (EMT).