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Related Concept Videos

¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved in...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...

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Updated: May 14, 2026

Disentangling Glycan-Protein Interactions: Nuclear Magnetic Resonance (NMR) to the Rescue
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Monomer-collagen interactions studied by saturation transfer difference NMR.

N Hiraishi1, N Tochio, T Kigawa

  • 1Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan. hiraope@tmd.ac.jp

Journal of Dental Research
|January 24, 2013
PubMed
Summary

The study reveals that 10-methacryloyloxydecyl dihydrogenphosphate (MDP) interacts strongly with collagen, unlike 4-methacryloyloxyethyl trimellitate anhydride (4-META). This MDP-collagen interaction is key to achieving stable dentin bonding in dental adhesives.

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Published on: September 20, 2012

Area of Science:

  • Biomaterials science
  • Dental materials research
  • Molecular interactions

Background:

  • Functional monomers in dentin adhesives are crucial for dental substrate wetting and mineralization.
  • The molecular-level interaction between these monomers and collagen remains poorly understood.

Purpose of the Study:

  • To investigate the binding interactions of 4-methacryloyloxyethyl trimellitate anhydride (4-META) and 10-methacryloyloxydecyl dihydrogenphosphate (MDP) with atelocollagen.
  • To elucidate the molecular mechanisms underlying stable dentin bonding.

Main Methods:

  • Saturation Transfer Difference (STD) NMR spectroscopy was employed.
  • Atelocollagen served as a triple-helical peptide model for collagen.

Main Results:

  • High STD intensities were observed for MDP on aliphatic protons, indicating interaction with collagen.
  • 4-META did not show significant STD intensities, suggesting a weaker interaction.
  • Hydrophobic interactions between MDP and collagen were implied by the STD results.

Conclusions:

  • MDP exhibits a stable interaction with collagen, likely due to hydrophobic forces.
  • MDP-collagen complexation is proposed as a mechanism for robust dentin adhesion.
  • Understanding these interactions can guide the development of improved dental adhesives.