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Related Experiment Video

Updated: May 14, 2026

Genetic Profiling and Genome-Scale Dropout Screening to Identify Therapeutic Targets in Mouse Models of Malignant Peripheral Nerve Sheath Tumor

Genetic Profiling and Genome-Scale Dropout Screening to Identify Therapeutic Targets in Mouse Models of Malignant Peripheral Nerve Sheath Tumor

Published on: August 25, 2023

Personalized chemotherapy profiling using cancer cell lines from selectable mice.

Hirohiko Kamiyama¹, Sherri Rauenzahn, Joong Sup Shim

¹The Sol Goldman Pancreatic Cancer Research Center, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA.

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research

|January 24, 2013

Summary

This summary is machine-generated.

Generating pure cancer cell lines from patient tumors is now possible using novel mouse models. This advance enables high-throughput drug screening for personalized chemotherapy, identifying effective treatments for individual patients.

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Last Updated: May 14, 2026

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Published on: February 27, 2015

Area of Science:

Oncology
Genetics
Drug Discovery

Background:

Generating patient-specific cancer cell lines for drug testing is challenging due to stromal cell contamination.
High-throughput chemosensitivity assays are crucial for prioritizing chemotherapy agents.

Purpose of the Study:

To develop a method for producing pure human cancer cell lines from primary tumors.
To enable high-throughput drug screening for personalized cancer therapy.

Main Methods:

Developed hypoxanthine phosphoribosyl transferase (hprt)-null immunodeficient mice.
Utilized biochemical selection to replace murine stromal cells with human cancer cells.
Explanted pancreatic and ovarian cancers for cell line derivation and drug screening.

Main Results:

Successfully generated pure human cancer cell lines from mouse-xenografted tumors.
Screened one cell line against 3,131 drugs, identifying 77 FDA-approved drugs with activity.
Discovered two novel drugs to which the cancer cell line showed unique sensitivity.

Conclusions:

Patient-specific cell lines can be derived using biochemically selectable mice.
This method facilitates personalized chemotherapy through targeted drug selection.
The approach holds promise for advancing precision oncology.