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Updated: May 14, 2026

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
Published on: June 23, 2016
High density DNA loading on the M13 bacteriophage provides access to colorimetric and fluorescent protein microarray
Dylan W Domaille1, Ju Hun Lee, Jennifer N Cha
1Department of Chemical and Biological Engineering, University of Colorado, Boulder, Boulder, CO, USA.
Abstract:
We report the synthesis, characterization, and protein sensing capabilities of M13 bacteriophage-DNA bioconjugates. DNA oligonucleotides were conjugated to M13 through acyl hydrazone linkages. In one case, DNAzymes retained their catalytic ability when anchored to the virus coat, and in a separate study, the dynamic nature of the hydrazone allowed for liberation of DNA from the phage under mild conditions.
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