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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

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Updated: May 14, 2026

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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RNA interference--a silent but an efficient therapeutic tool.

Puthucode Venkatakrishnan Ramachandran1, Savarimuthu Ignacimuthu

  • 1Entomology Research Institute, Loyola College, Chennai, Tamilnadu, India.

Applied Biochemistry and Biotechnology
|January 24, 2013
PubMed
Summary

RNA interference (RNAi) offers efficient gene silencing for therapeutic applications. This gene regulation pathway utilizes small interfering RNAs (siRNAs) to target and downregulate specific gene expression, aiding in disease treatment.

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Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Biotechnology

Background:

  • RNA interference (RNAi) is a conserved biological pathway regulating gene expression.
  • RNAi provides efficient gene downregulation by targeting complementary RNA transcripts.
  • It offers advantages over other antisense-based therapeutic techniques.

Purpose of the Study:

  • To review the therapeutic applications of RNA interference.
  • To highlight RNAi's role in treating debilitating human diseases.

Main Methods:

  • Utilizing chemically synthesized small interfering RNAs (siRNAs).
  • Employing plasmid or viral vectors for sustained siRNA delivery.
  • Application in reverse genetics for gene function studies.

Main Results:

  • RNAi enables effective gene silencing for research and therapy.
  • Vector-based delivery enhances knockdown effect stability.
  • Demonstrated potential as a therapeutic tool for various diseases.

Conclusions:

  • RNA interference is a powerful tool in molecular biology and medicine.
  • Its therapeutic potential against debilitating diseases is significant and warrants further investigation.