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High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization
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Fluorogenic label to quantify the cytosolic delivery of macromolecules

Tzu-Yuan Chao1, Ronald T Raines

  • 1Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706, USA.

Molecular Biosystems
|January 24, 2013
PubMed
Summary

Researchers developed a novel method to track macromolecule delivery into human cells. This technique uses a fluorescent probe and enzyme activity to quantify cytosolic entry, successfully measuring ribonuclease A delivery.

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