Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mechanism of Angiogenesis

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Patient-reported outcomes and comprehensive assessment of wrist function after scaphoid reconstruction: A single-center retrospective study on 162 patients.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2024

Same author

Postoperative scaphoid alignment, smoking, and avascular necrosis mostly influence union rate after scaphoid reconstruction: Results from a retrospective single center study involving 370 patients.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2023

Same author

An algorithmic diagnostic approach to scapholunate ligament injuries based on comparison of X-ray examinations and arthroscopy in 414 patients.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2022

Same author

Vascular malformations of upper and lower extremity - from radiological interventional therapy to surgical soft tissue reconstruction - an interdisciplinary treatment.

Clinical hemorheology and microcirculation·2017

Same author

New reconstruction for bone integration of non-vascularized autogenous bone graft with better bony union and revascularisation.

Archives of orthopaedic and trauma surgery·2017

Same author

The protective effect of human renal sinus fat on glomerular cells is reversed by the hepatokine fetuin-A.

Scientific reports·2017

Search research articles

Related Experiment Video

Updated: May 14, 2026

Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

Published on: February 3, 2023

[Angiogenesis and vascularisation in adipose tissue engineering].

J H Dolderer¹, F Medved, R M Haas

¹Hochschulzentrum für Plastische, Hand- und Wiederherstellungschirurgie, -Universitätsklinikum Regensburg, Regensburg. drdolderer@hotmail.com

Handchirurgie, Mikrochirurgie, Plastische Chirurgie : Organ Der Deutschsprachigen Arbeitsgemeinschaft Fur Handchirurgie : Organ Der Deutschsprachigen Arbeitsgemeinschaft Fur Mikrochirurgie Der Peripheren Nerven Und Gefasse : Organ Der V

|January 24, 2013

Summary

Reconstructing large soft tissue defects requires new methods. Tissue engineering aims to create stable, autologous adipose tissue grafts, focusing on vascularization for long-term survival and volume maintenance.

More Related Videos

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Published on: April 30, 2017

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Published on: May 30, 2016

Related Experiment Videos

Last Updated: May 14, 2026

Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

Published on: February 3, 2023

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Published on: April 30, 2017

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Published on: May 30, 2016

Area of Science:

Regenerative Medicine
Biomaterials Science
Tissue Engineering

Context:

Current soft tissue reconstruction methods like microsurgical tissue transfer have limitations and complications.
Significant need exists for permanent soft tissue replacement using autologous tissues.
Autologous adipose tissue transplantation shows promise but faces challenges with graft survival and volume stability.

Purpose:

To explore tissue engineering strategies for producing stable, autologous adipose tissue for transplantation.
To investigate the critical role of vascularization in the quality, volume, and long-term survival of engineered adipose tissue.
To understand the relationship between adipogenesis regulation and angiogenesis in engineered tissue constructs.

Summary:

Microsurgical tissue transfer is standard for large defects but has limitations.
Autologous fat grafting often results in volume loss due to poor graft survival.
Tissue engineering offers a solution by creating vascularized adipose tissue constructs for permanent replacement.

Impact:

Development of improved methods for soft tissue reconstruction.
Potential for enhanced patient outcomes in reconstructive surgery.
Advancement of tissue engineering applications in regenerative medicine.