Cytogenetic characterization of two human milk-derived cell line (HBL-100) passages differing in tumorigenicity

Using the trypsin-Giemsa staining technique, cytogenetic analysis was done to determine the karyotypic characteristics in cells from the early nontumorigenic passage 12 and the late tumorigenic passage 147 of the human breast milk-derived cell line HBL-100. Acquisition of exclusive marker chromosomes in the late passage of HBL-100 may be responsible for the tumorigenic behavior of these cells in athymic nude mice.