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Related Concept Videos

The Blood-brain Barrier00:49

The Blood-brain Barrier

Overview

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Related Experiment Video

Updated: May 14, 2026

Use of the MicroSiM (µSiM) Barrier Tissue Platform for Modeling the Blood-Brain Barrier
09:10

Use of the MicroSiM (µSiM) Barrier Tissue Platform for Modeling the Blood-Brain Barrier

Published on: January 12, 2024

SyM-BBB: a microfluidic Blood Brain Barrier model.

Balabhaskar Prabhakarpandian1, Ming-Che Shen, Joseph B Nichols

  • 1Biomedical Technology, CFD Research Corporation, Huntsville, AL 35805, USA. bxp@cfdrc.com

Lab on a Chip
|January 25, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a microfluidic Synthetic Microvasculature model of the Blood-Brain Barrier (SyM-BBB). This innovative platform overcomes limitations of traditional models, enabling better study of barrier function and drug delivery.

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Last Updated: May 14, 2026

Use of the MicroSiM (µSiM) Barrier Tissue Platform for Modeling the Blood-Brain Barrier
09:10

Use of the MicroSiM (µSiM) Barrier Tissue Platform for Modeling the Blood-Brain Barrier

Published on: January 12, 2024

A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain
07:52

A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

Published on: April 9, 2019

A Triple Culture Cell System Modeling the Human Blood-Brain Barrier
09:21

A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

Published on: November 30, 2021

Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Pharmacology

Background:

  • Current Blood-Brain Barrier (BBB) models using Transwell chambers lack critical microenvironmental factors like shear stress and real-time visualization.
  • These conventional methods are costly due to high consumable requirements and do not accurately mimic in vivo conditions.

Purpose of the Study:

  • To develop and validate a novel microfluidics-based Synthetic Microvasculature model of the Blood-Brain Barrier (SyM-BBB).
  • To overcome the limitations of existing BBB models, offering a more physiologically relevant and efficient platform for research.

Main Methods:

  • The SyM-BBB platform utilizes a disposable, optically clear microfluidic chip with a two-compartment chamber for cell culture.
  • Endothelial cells (RBE4) were cultured on the apical side, with Astrocyte Conditioned Media (ACM) in the basolateral compartment.
  • Biochemical and permeation studies were conducted to assess BBB integrity and function.

Main Results:

  • Biochemical analysis confirmed the upregulation of tight junction molecules, indicating a robust barrier.
  • Permeation studies demonstrated an intact BBB, validating the model's structural integrity.
  • Successful demonstration of a transporter assay confirmed the functional capacity of the SyM-BBB model.

Conclusions:

  • The SyM-BBB platform provides a superior microfluidic model for studying the Blood-Brain Barrier.
  • This model effectively replicates key physiological parameters, offering enhanced capabilities for research into BBB permeability and function.