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Related Experiment Video

Updated: May 14, 2026

Characterizing Mammalian Zinc Transporters Using an In Vitro Zinc Transport Assay
07:55

Characterizing Mammalian Zinc Transporters Using an In Vitro Zinc Transport Assay

Published on: June 2, 2023

Long wavelength fluorescence ratiometric zinc biosensor.

Hui Hui Zeng1, Evgenia G Matveeva, Andrea K Stoddard

  • 1Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

Journal of Fluorescence
|January 25, 2013
PubMed
Summary

A novel protein-based biosensor accurately quantifies free zinc ions in solution at picomolar levels. This ratiometric fluorescence sensor offers high specificity and is suitable for complex biological samples.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Accurate quantification of free zinc ions is crucial in biological and environmental studies.
  • Existing methods for zinc ion detection can lack sensitivity or specificity.
  • Ratiometric fluorescence measurements offer enhanced accuracy and are compatible with advanced microscopy techniques.

Purpose of the Study:

  • To develop a protein-based ratiometric fluorescence biosensor for sensitive detection of free zinc ions.
  • To achieve picomolar sensitivity and high specificity for zinc ion recognition.
  • To enable zinc ion quantitation in complex matrices with minimal background interference.

Main Methods:

  • Engineering a protein-based fluorescence biosensor utilizing apo-carbonic anhydrase for zinc ion recognition.

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Last Updated: May 14, 2026

Characterizing Mammalian Zinc Transporters Using an In Vitro Zinc Transport Assay
07:55

Characterizing Mammalian Zinc Transporters Using an In Vitro Zinc Transport Assay

Published on: June 2, 2023

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels
10:07

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

Published on: January 27, 2013

  • Employing ratiometric fluorescence measurements for signal quantification.
  • Utilizing longer emission wavelengths to minimize background fluorescence in biological samples.

    • Main Results:

    • The biosensor demonstrates high sensitivity, detecting free zinc ions down to picomolar concentrations.
    • Ratiometric measurements ensure accurate quantitation, suitable for laser scanning microscopy.
    • The sensor exhibits high and controllable specificity for zinc ion binding.
    • The use of longer emission wavelengths effectively reduces background fluorescence in complex matrices.

    Conclusions:

    • A novel, highly sensitive, and specific protein-based ratiometric fluorescence biosensor for free zinc ions has been developed.
    • This biosensor is well-suited for accurate zinc ion quantitation in biological tissues and other challenging samples.
    • The technology holds promise for advancing research in fields requiring precise zinc ion monitoring.