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Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
Sequence conservation of the region targeted by the Abbott RealTime HBV viral load assay in clinical specimens
Gavin A Cloherty1, James Rhoads, Thomas P Young
1Abbott Molecular, Des Plaines, Illinois, USA.
Journal of Clinical Microbiology
|January 25, 2013
Abstract:
The Abbott RealTime HBV assay targets the N-terminal region of the S gene. Here we analyzed the sequence variability of the assay target region from >2,100 clinical specimens. Thermodynamic modeling of the percentage of bound primer/probe at the assay annealing temperature was performed to assess the potential effect of sequence variability.
