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Published on: October 21, 2010
Microplate alamarBlue assay for Paracoccidioides susceptibility testing
A C A de Paula e Silva1, H C Oliveira, J F Silva
1Faculdade de Ciências Farmacêuticas, UNESP-Universidade Estadual Paulista, Araraquara, Departamento de Analises Clinicas, Laboratório de Micologia Clínica, São Paulo, Brazil.
Abstract:
CLSI method M27-A3 is not available for use with dimorphic fungi, such as those of the Paracoccidioides genus. In this study, we developed a microdilution method and added the alamarBlue reagent to test the responses of Paracoccidioides brasiliensis and Paracoccidioides lutzii against amphotericin B and itraconazole antifungals. The test proved to be sensitive, practical, and inexpensive and can be used to monitor the activity of low-growth microorganisms and their response to various drugs.
