Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Bacteriophages

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

Transformation

Transformation

Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...

PCR

PCR

DNA Topoisomerases

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. Type I...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Naltrexone modulates contextual processing in depression.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2020

Same author

Non-vitamin K antagonist oral anticoagulants (NOACs) for thromboembolic prevention, are they safe in congenital heart disease? Results of a worldwide study.

International journal of cardiology·2019

Same author

Evaluation of microbial diversity in the pilot-scale beer brewing process by culture-dependent and culture-independent method.

Journal of applied microbiology·2015

Same author

Damage to the mandible induced by radiation therapy of intraoral cancer: a microradiographic study.

Dento maxillo facial radiology·2015

Same author

Evaluation of microbial diversity in the pilot-scale beer brewing process by culture-dependent and culture-independent method.

Journal of applied microbiology·2014

Same author

Volatile compounds and the changes in their concentration levels during storage in beers containing varying malt concentrations.

Journal of food science·2010

Search research articles

Related Experiment Video

Updated: May 14, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Manipulation of a Large DNA Molecule using the Phase Transition.

A Mizuno¹, S Katsura

¹Department of Ecological Engineering, Toyohashi University of Technology, Tempaku-cho, Toyohashi, Aichi, 441-8580 Japan.

Journal of Biological Physics

|January 25, 2013

Summary

This summary is machine-generated.

Researchers developed a new method to sequence long DNA molecules by cutting fragments from the ends, preserving order information. This technique manipulates genomic DNA into a globular form for easier handling and precise cutting using electrochemically supplied magnesium ions.

Keywords:

DNA globular transformation laser trap local reaction manipulation single molecule stretching

More Related Videos

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Chemical Dimerization-Induced Protein Condensates on Telomeres

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

Related Experiment Videos

Last Updated: May 14, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Chemical Dimerization-Induced Protein Condensates on Telomeres

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Conventional DNA sequencing methods are limited to short fragments (up to 1000 base pairs).
Sequencing long DNA requires fragmentation, leading to loss of order information.
Preparing fragments from the terminus of long DNA can preserve order.

Purpose of the Study:

To develop a method for preparing DNA fragments from the terminus of long DNA molecules.
To enable DNA sequencing with preserved order information.
To improve manipulation and cutting of large genomic DNA.

Main Methods:

Genomic DNA manipulation using reversible globular transformation for stability and handling.
Sequential spinning of coiled DNA from globular form.
Fixation of stretched DNA onto a glass surface in a desired pattern.
Development of a method for cutting DNA from the terminus of stretched molecules.
Electrochemical supply of magnesium ions to activate restriction enzymes for precise DNA cutting.

Main Results:

Successful manipulation and stabilization of large genomic DNA via globular transformation.
Demonstrated sequential spinning and fixation of DNA onto a glass surface.
Developed and validated a method for cutting DNA fragments from stretched molecules.
Achieved targeted DNA cleavage using electrochemically controlled restriction enzyme activation.

Conclusions:

The developed method allows for the preparation of DNA fragments from the terminus of long DNA molecules, preserving their order.
This technique overcomes limitations of conventional DNA sequencing by enabling manipulation and precise cutting of large DNA.
The approach holds potential for advancing genomic studies requiring long-read sequencing with positional information.