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Related Experiment Video

Updated: May 14, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Published on: August 11, 2020

Single molecule probe scanning optical force imaging microscopefor viewing live cells.

K Ritchie, A Kusumi

Journal of Biological Physics

|January 25, 2013

Summary

This summary is machine-generated.

We developed a new imaging system to track forces on live cell membranes. This method uses a gold nanoparticle probe and an optical trap to reveal membrane barrier structures.

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Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

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Published on: July 10, 2019

Area of Science:

Biophysics
Cell Biology
Nanotechnology

Background:

Understanding forces within live cells is crucial for cell function.
Existing methods lack the sensitivity to probe nanoscale forces in real-time.

Purpose of the Study:

To develop a novel imaging system for mapping forces on/in live cells.
To utilize single molecule probes for high-resolution force imaging.

Main Methods:

Combined optical trapping with single particle tracking.
Used a single gold nanoparticle (40 nm) conjugated to a molecule of interest as a probe.
Synchronized cell scanning with probe position capture via computer control.

Main Results:

Successfully imaged forces and barriers within live cell membranes.
Demonstrated the system's ability to track probe movement against cellular resistance.
Revealed fine structural details of membrane barriers.

Conclusions:

The developed system provides a sensitive method for imaging cellular forces.
This technique offers new insights into membrane biophysics and molecular interactions.