Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Changes in blood pressure among students attending Glasgow University between 1948 and 1968: analyses of cross sectional surveys.

BMJ (Clinical research ed.)·2001
Same author

Smoking in adolescence and young adulthood and mortality in later life: prospective observational study.

Journal of epidemiology and community health·2001
Same author

Social circumstances in childhood and cardiovascular disease mortality: prospective observational study of Glasgow University students.

Journal of epidemiology and community health·2001
Same author

Reflections on the limitations to epidemiology.

Journal of clinical epidemiology·2001
Same author

Myasthenia gravis, corticosteroids and osteoporosis prophylaxis.

Journal of neurology·2001
Same author

Dietary fat intake and prevention of cardiovascular disease: systematic review.

BMJ (Clinical research ed.)·2001

Related Experiment Video

Updated: May 14, 2026

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

Synchrotron-based Biological Microspectroscopy: From the Mid-Infrared through the Far-Infrared Regimes.

L M Miller1, G D Smith, G L Carr

  • 1Brookhaven National Laboratory, National Synchrotron Light Source, Upton, NY 11973 USA.

Journal of Biological Physics
|January 25, 2013
PubMed
Summary
This summary is machine-generated.

Synchrotron infrared radiation enables high-resolution molecular spectroscopy for disease detection. This advanced technique analyzes mid- and far-infrared spectra to identify subtle chemical changes in complex molecules.

Keywords:
Biological microspectroscopyTHz microscopyfar-infraredmid-infraredsynchrotron radiation

More Related Videos

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
07:38

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

Related Experiment Videos

Last Updated: May 14, 2026

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
07:38

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

Area of Science:

  • Spectroscopy
  • Molecular Biology
  • Biophysics

Background:

  • Synchrotron storage rings provide a high-brightness infrared radiation source.
  • Infrared microspectroscopy is valuable for analyzing molecular vibrations.

Purpose of the Study:

  • To demonstrate the utility of synchrotron infrared radiation for diffraction-limited microspectroscopy.
  • To explore the application of mid- and far-infrared spectroscopy in disease-associated chemical change detection.

Main Methods:

  • Utilized synchrotron storage rings for generating infrared radiation.
  • Performed diffraction-limited microspectroscopy across mid- and far-infrared ranges.
  • Investigated molecular vibrational modes sensitive to chemical alterations.

Main Results:

  • Demonstrated high-brightness infrared source for microspectroscopy.
  • Showcased sensitivity of mid-infrared absorption to small chemical changes linked to diseases.
  • Indicated far-infrared modes relate to folding/twisting of large molecules.
  • Successfully performed microscopy at 1 THz frequency.

Conclusions:

  • Synchrotron infrared microspectroscopy is a powerful tool for molecular analysis.
  • The technique can detect disease-specific molecular signatures.
  • Further research into far-infrared spectroscopy may reveal insights into macromolecular structure.