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Subcellular Fractionation

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

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Related Experiment Video

Updated: May 14, 2026

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Published on: September 2, 2013

Proteomics sample preparation, preservation, and fractionation

Gary B Smejkal¹

¹Covaris Inc., 14 Gill Street, Suite H, Woburn, MA 01801, USA ; Hubbard Center for Genome Studies, University of New Hampshire, Durham, NH, USA.

International Journal of Proteomics

|January 25, 2013

Summary

No abstract available in PubMed .

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