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A novel reversed-phase LC method for quantitative detection of azithromycin in bulk drug and tablet formulations in
R W Odendaal1, W Liebenberg, M E Aucamp
1Unit for Drug Research and Development, Faculty of Health Sciences, North-West University, Potchefstroom, South Africa.
Abstract:
A novel reversed-phase HPLC method with UV-detection for separation, identification and quantification of azithromycin (AZH) was developed. The method was validated for the purpose of quantifying AZH in stability, dissolution and solubility studies. The method was validated at two optimum wavelengths where linear regression at both 205 and 210 nm, resulted in correlation coefficients of r2 = 0.9999. This HPLC method proved to be superior to other published methods due to the specificity, resulting in less peak interference and tailing. The pH of the mobile phase, as well as the ratio of buffer to organic component included in the mobile phase, proved to be critical factors in the improved detection of AZH.
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